H19-DMR FISH Probe
The H19-DMR FISH probe is designed to hybridize to the H19-DMR gene and is primarily used for detecting amplifications and deletions associated with the gene. This probe is FISH confirmed on normal peripheral blood metaphase spreads and interphase nuclei. The probe can be labeled in one of five colors. Each probe is sold in a 20 test kit (approximately 20 slides - 22x22 mm area) and includes hybridization buffer. Please note that due to design optimizations, prices are subject to change.
** This product is for in vitro and research use only. This product is not intended for diagnostic use.
| SKU | Test Kits | Buffer | Dye Color | Order Now |
|---|---|---|---|---|
| H19-ICR-20-OR (Standard Design) | 20 (40 μL) | 200 μL |  |
|
| H19-ICR-20-RE | 20 (40 μL) | 200 μL |  |
|
| H19-ICR-20-GO | 20 (40 μL) | 200 μL |  |
|
| H19-ICR-20-GR | 20 (40 μL) | 200 μL |  |
|
| H19-ICR-20-AQ | 20 (40 μL) | 200 μL |  |
|
| H19-ICR-20-DIG | 20 (40 μL) | 200 μL |  |
|
| H19-ICR-20-BIO | 20 (40 μL) | 200 μL |  |
Gene Summary
This region represents a methylation-sensitive enhancer-blocking element that controls imprinted expression of the non-coding H19 gene and the gene encoding insulin-like growth factor 2 (IGF2). These neighboring genes exist in a head-to-tail arrangement in opposite orientations and share an enhancer, but the H19 gene is only expressed from the maternal allele, while the IGF2 gene is only expressed from the paternal allele. This element, which is a differentially methylated region (DMR), is located just upstream of the H19 gene. It is unmethylated on the maternal allele, which permits binding of the CTCF protein, and it can thus function as an enhancer-blocking element to prevent activation of IGF2 by the enhancer, thereby allowing H19 activation. However, it is methylated on the paternal allele and CTCF cannot bind, thus allowing the enhancer to activate the IGF2 gene, and the H19 gene is silenced. This DMR includes multiple direct repeat units and seven CTCF-binding sites. Mutations in this element are a cause of Wilms tumor, and also Beckwith-Wiedemann syndrome through either a gain or loss of methylation. This element has also been implicated in dysregulated H19-IGF2 imprinting found in osteosarcoma and in Silver-Russell syndrome. [provided by RefSeq, Feb 2015]
Gene Details
Gene Symbol: H19-ICR
Gene Name: H19/IGF2 Imprinting Control Region
Locus: 11p15.5
FISH Probe Protocols
| Protocol, Procedure, or Form Name | Last Modified | Download |
|---|
Customer Publications
Product Details
Product: H19-DMR FISH Probe
Test Kits: 20 (40 μL)
ISH Buffer: 200 μL
SKU: H19-ICR-20-OR
Material Safety Data Sheet: MSDS.pdf
Turnaround Time: 7-10 Business Days
Shipping Time: 1-2 Day Expedited Shipping