ALK FISH Probe

The ALK FISH probe is designed to hybridize to the ALK gene and is primarily used for detecting amplifications and deletions associated with the gene. This probe is FISH confirmed on normal peripheral blood metaphase spreads and interphase nuclei. The probe can be labeled in one of five colors. Each probe is sold in a 20 test kit (approximately 20 slides - 22x22 mm area) and includes hybridization buffer. Please note that due to design optimizations, prices are subject to change.

** This product is for in vitro and research use only. This product is not intended for diagnostic use.

Turnaround Time: 7-10 Business Days Shipping Time: 1-2 Day Expedited Shipping

Download Datasheet Download FISH Protocol Download Material Safety Data Sheet

SKU	Test Kits	Buffer
ALK-20-OR (Standard Design)	20 (40 μL)	200 μL
ALK-20-RE	20 (40 μL)	200 μL
ALK-20-GO	20 (40 μL)	200 μL
ALK-20-GR	20 (40 μL)	200 μL
ALK-20-AQ	20 (40 μL)	200 μL
ALK-20-DIG	20 (40 μL)	200 μL
ALK-20-BIO	20 (40 μL)	200 μL

Gene Summary

This gene encodes a receptor tyrosine kinase, which belongs to the insulin receptor superfamily. This protein comprises an extracellular domain, an hydrophobic stretch corresponding to a single pass transmembrane region, and an intracellular kinase domain. It plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. This gene has been found to be rearranged, mutated, or amplified in a series of tumours including anaplastic large cell lymphomas, neuroblastoma, and non-small cell lung cancer. The chromosomal rearrangements are the most common genetic alterations in this gene, which result in creation of multiple fusion genes in tumourigenesis, including ALK (chromosome 2)/EML4 (chromosome 2), ALK/RANBP2 (chromosome 2), ALK/ATIC (chromosome 2), ALK/TFG (chromosome 3), ALK/NPM1 (chromosome 5), ALK/SQSTM1 (chromosome 5), ALK/KIF5B (chromosome 10), ALK/CLTC (chromosome 17), ALK/TPM4 (chromosome 19), and ALK/MSN (chromosome X).[provided by RefSeq, Jan 2011]

Gene Details

Gene Symbol: ALK

Gene Name: ALK Receptor Tyrosine Kinase

Chromosome: CHR2: 29415639-30144477

Locus: 2p23.2-p23.1

FISH Probe Protocols

Protocol, Procedure, or Form Name	Last Modified	Download

Atypical Spitzoid Neoplasms in Childhood: A Molecular and Outcome Study

Atypical spitzoid neoplasms (APNs) are primarily pediatric lesions characterized by their intermediate features; clinically and histopathologically, they fall somewhere between benign spitz nevi and malignant melanoma. The genetics of these tumors are still poorly understood. In this study, 34 APNs were analyzed using FISH and IHC. Our ALK, BRAF, and NTRK1 break-apart FISH probes were used to detect rearrangements of the genes .

Alterations in ALK/ROS1/NTRK/MET drive a group of infantile hemispheric gliomas

While studies are plentiful on adult gliomas, infant cases are historically understudied. This team sought to account for that lack of data by analyzing glioma biopsies from 150 infants. As part of genetic profiling, our ALK break apart probe was used to detect ALK rearrangements. The team was able to divide the tumors into three genetic subtypes that were tightly tied to clinical outcome. They also found that many of the tumors harbored just a single oncogene, evidence that infant gliomas are usually single driver tumors.

ALK-rearranged renal cell carcinomas in Polish population

Since the first report 2010, 22 cases of ALK-rearranged renal cell carcinoma (RCC) have been described. This study screened over 1000 Polish RCC patients for ALK translocations using IHC followed by FISH with our ALK break apart FISH probe. Only 31 cases were considered potentially positive or indeterminable by IHC; of these, none tested positive for ALK rearrangement with FISH. These results suggests that the mutation might be related to ethnicity, warranting further investigation into its prevalence in other ethnicities.

Product Details

Product: ALK FISH Probe

Test Kits: 20 (40 μL)

ISH Buffer: 200 μL

SKU: ALK-20-OR

Material Safety Data Sheet: MSDS.pdf

Turnaround Time: 7-10 Business Days

Shipping Time: 1-2 Day Expedited Shipping

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